Low temperature is a significant abiotic stress that critically limits mangrove productiveness and distribution. Kandelia obovata is probably the most cold-resistance specie in mangrove vegetation, however little is understood concerning the molecular mechanism underlying its resistance to chilly. Osmotin is a key protein related to abiotic and biotic stress response in vegetation however no details about this gene in Ok. obovata was reported.
On this research, a cDNA sequence encoding osmotin, KoOsmotin (GenBank accession no. KP267758), was cloned from mangrove plant Ok. obovata. The KoOsmotin protein was composed of 221 amino acids and confirmed a calculated molecular mass of 24.11 kDa with pI 4.92. The KoOsmotin contained sixteen cysteine residues and an N-terminal sign peptide, which had been frequent signatures to most osmotins and pathogenesis-related 5 proteins. The three-dimensional (3D) mannequin of KoOsmotin, contained one α-helix and eleven β-strands, was fashioned by three attribute domains. Database comparisons of the KoOsmotin confirmed the closest identification (55.75%) with the osmotin 34 from Theobroma cacao. The phylogenetic tree additionally revealed that the KoOsmotin was clustered within the department of osmotin/OLP (osmotin-like protien). The KoOsmotin protein was proved to be localized to each the plasma membrane and cytoplasm by the subcellular localization evaluation. Gene expression confirmed that the KoOsmotin was induced primarily and extremely within the leaves of Ok. obovata, however much less abundantly in stems and roots. The overexpressing of KoOsmotin conferred chilly tolerance in Escherichia coli cells.
CRISPR-Cas9 multiplex genome modifying of the hydroxyproline-O-galactosyltransferase gene household alters arabinogalactan-protein glycosylation and performance in Arabidopsis
Arabinogalactan-proteins (AGPs) are a category of hydroxyproline-rich proteins (HRGPs) which are closely glycosylated (> 90%) with kind II arabinogalactans (AGs). AGPs are implicated in varied plant progress and growth processes together with cell growth, somatic embryogenesis, root and stem progress, salt tolerance, hormone signaling, female and male gametophyte growth, and protection. Up to now, eight Hyp-O-galactosyltransferases (GALT2-6, HPGT1-3) have been recognized; these enzymes are answerable for including the primary sugar, galactose, onto AGPs. Resulting from gene redundancy among the many GALTs, single or double galt genetic knockout mutants are sometimes not ample to totally reveal their organic features.
Right here, we report the profitable utility of CRISPR-Cas9 gene modifying/multiplexing know-how to generate higher-order knockout mutants of 5 members of the GALT gene household. AGPs evaluation of higher-order galt mutants demonstrated considerably much less glycosylated AGPs in rosette leaves, stems, and siliques in comparison with the corresponding wild-type organs. Monosaccharide composition evaluation of AGPs remoted from rosette leaves revealed vital decreases in arabinose and galactose in all of the higher-order galt mutants. Phenotypic analyses revealed that mutation of two or extra GALT genes was capable of overcome the expansion inhibitory impact of β-D-Gal-Yariv reagent, which particularly binds to β-1,3-galactan backbones on AGPs. As well as, the galt2 galt3 galt4 galt5 gal6 mutant exhibited decreased general progress, impaired root progress, irregular pollen, shorter siliques, and decreased seed set. Reciprocal crossing experiments demonstrated that galt2 galt3 galt4 galt5 gal6 mutants had defects within the feminine gametophyte which had been answerable for decreased seed set.
These lesions subsequently unfold and turned a darker brown. The leaves of the affected vegetation turned yellow and wilted. Because the illness progressed the vegetation finally died. Years of rising cucumbers and ample soil moisture within the greenhouse, may need led to a illness incidence of roughly 7%. Symptomatic tissue items had been floor disinfested in 2% sodium hypochlorite for 60 s, rinsed thrice in sterile water, and subsequently plated on potato dextrose agar (PDA) incubated at 25°C .
Lignin composition and timing of cell wall lignification are concerned in Brassica napus resistance to Sclerotinia sclerotiorum stem rot
Sclerotinia stem rot (SSR) is an economically and globally vital illness in oilseed rape (Brassica napus L.) attributable to the necrotrophic ascomycete Sclerotinia sclerotiorum. This research explored the function of cell wall reinforcement by lignin as a related issue for efficient plant defence towards assault of this pathogen. Expression of key genes within the phenylpropanoid pathway and the induced synthesis of lignin in contaminated stem tissues had been investigated in a research evaluating a prone (Loras) and a reasonably resistant cultivar. Knowledge revealed an earlier and extra fast defence activation in ZY821 via up-regulation of transcript ranges of genes associated to key steps within the phenylpropanoid pathway related to enhanced lignin deposition within the resistant B. napus genotype.
Expression stage of BnCAD5, encoding a cinnamyl alcohol dehydrogenase, answerable for conversion of monolignol to lignin, was extra quickly up-regulated in ZY821 than Loras. The same expression sample of BnCAD5 and the gene BnF5H, encoding for the ferulate-5-hydroxylase which catalyses the synthesis of syringyl (S) lignin precursors, means that BnCAD5 is concerned within the S lignin formation. Histological observations confirmed these outcomes, exhibiting an earlier improve of S lignin deposition within the contaminated resistant genotype. Deposition of guaiacyl (G) lignin was detected in each genotypes and is thus thought-about a part of basal, cultivar-independent defence response of B. napus to stem rot.
 Eva QPCR SuperMix Kit |
|
K5052200 |
Biochain |
200 reactions |
EUR 188 |
Eva QPCR SuperMix Kit |
|
K5052400 |
Biochain |
400 reactions |
EUR 316 |
 Fast Eva QPCR SuperMix Kit |
|
K5052002 |
Biochain |
200 rxn |
EUR 188 |
 Pro QPCR SuperMix Kit |
|
K5053200 |
Biochain |
200 reactions |
EUR 161 |
Pro QPCR SuperMix Kit |
|
K5053400 |
Biochain |
400 reactions |
EUR 291 |
 Green qPCR SuperMix |
|
20-abx098031 |
Abbexa |
-
EUR 1245.60
-
EUR 526.80
-
EUR 760.80
|
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 Probe qPCR SuperMix |
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20-abx098040 |
Abbexa |
-
EUR 1245.60
-
EUR 526.80
-
EUR 760.80
|
|
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 Pro QPCR SuperMix Kit - ROX premixed |
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K5056200 |
Biochain |
200 reactions |
EUR 161 |
Pro QPCR SuperMix Kit - ROX premixed |
|
K5056400 |
Biochain |
400 reactions |
EUR 291 |
 Green qPCR SuperMix UDG |
|
20-abx098032 |
Abbexa |
-
EUR 1362.00
-
EUR 543.60
-
EUR 794.40
|
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 Top Green qPCR SuperMix |
|
20-abx098033 |
Abbexa |
-
EUR 994.80
-
EUR 260.40
-
EUR 1362.00
-
EUR 493.20
|
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 Tip Green qPCR SuperMix |
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20-abx098034 |
Abbexa |
-
EUR 1161.60
-
EUR 276.00
-
EUR 1596.00
-
EUR 560.40
|
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 Fast Pro QPCR SuperMix Kit - ROX premixed |
|
K5058200 |
Biochain |
200 reactions |
EUR 188 |
Fast Pro QPCR SuperMix Kit - ROX premixed |
|
K5058400 |
Biochain |
400 reactions |
EUR 316 |
 Library Quantification qPCR SuperMix |
|
20-abx098896 |
Abbexa |
|
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) Top Green qPCR SuperMix (+Dye II) |
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20-abx098890 |
Abbexa |
-
EUR 994.80
-
EUR 260.40
-
EUR 1362.00
-
EUR 493.20
|
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) Tip Green qPCR SuperMix (+Dye II) |
|
20-abx098891 |
Abbexa |
-
EUR 1161.60
-
EUR 276.00
-
EUR 1596.00
-
EUR 560.40
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 cDNA Synthesis SuperMix for qPCR |
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20-abx09801920ulSystems |
Abbexa |
|
- 100 rxns ×20 ul Systems
- 50 rxns × 20 ul Systems
|
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 HiScript II Q RT SuperMix for qPCR |
|
R222-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 282 |
) cDNA Synthesis SuperMix for qPCR (GC-rich) |
|
abx098024-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 861.6 |
|
|
 HiScript II Q Select RT SuperMix for qPCR |
|
R232-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 282 |
) HiScript III RT SuperMix for qPCR (+g DNA wiper) |
|
R323-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 351.6 |
 QCell-Eva One-Step qRT-PCR SuperMix Kit |
|
K5054004 |
Biochain |
400 rxn |
EUR 635 |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
|
K5054200 |
Biochain |
200 reactions |
EUR 418 |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
|
K5054400 |
Biochain |
400 reactions |
EUR 635 |
 Fast QCell-Eva One-Step qRT-PCR SuperMix Kit |
|
K5054002 |
Biochain |
200 rxn |
EUR 418 |
) HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) |
|
R233-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 292.8 |
 HiScript III All-in-one RT SuperMix Perfect for qPCR |
|
R333-01 |
Vazyme |
100 rxns (21 μl/rxn) |
EUR 174 |
 PCR SuperMix |
|
20-abx098887 |
Abbexa |
|
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) PCR SuperMix (+dye) |
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20-abx098003 |
Abbexa |
-
EUR 493.20
-
EUR 226.80
-
EUR 7408.80
-
EUR 309.60
|
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) PCR SuperMix (-dye) |
|
20-abx098002 |
Abbexa |
-
EUR 493.20
-
EUR 226.80
-
EUR 309.60
|
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 Plus PCR SuperMix |
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20-abx098888 |
Abbexa |
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 Evo? cDNA Supermix |
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M1168-100 |
Biovision |
each |
EUR 457.2 |
Evo? cDNA Supermix |
|
M1168-25 |
Biovision |
each |
EUR 320.4 |
 Novo? cDNA Supermix |
|
M1169-100 |
Biovision |
each |
EUR 529.2 |
Novo? cDNA Supermix |
|
M1169-25 |
Biovision |
each |
EUR 346.8 |
) T PCR SuperMix (-dye) |
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20-abx098005 |
Abbexa |
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) T PCR SuperMix (+dye) |
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20-abx098006 |
Abbexa |
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 cDNA Synthesis SuperMix |
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20-abx09801420ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
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) EasyPfu PCR SuperMix (-dye) |
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20-abx098009 |
Abbexa |
|
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) FastPfu PCR SuperMix (-dye) |
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20-abx098010 |
Abbexa |
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 Library Amplification SuperMix |
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20-abx098894 |
Abbexa |
|
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 QCell-Pro One-Step qRT-PCR SuperMix Kit |
|
K5055004 |
Biochain |
400 rxn |
EUR 609 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
|
K5055200 |
Biochain |
200 reactions |
EUR 381 |
QCell-Pro One-Step qRT-PCR SuperMix Kit |
|
K5055400 |
Biochain |
400 reactions |
EUR 609 |
) PCR SuperMix for PAGE (+dye) |
|
20-abx098004 |
Abbexa |
-
EUR 493.20
-
EUR 226.80
-
EUR 309.60
|
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 OneScriptcDNA Synthesis SuperMix |
|
G451 |
ABM |
25 x 20 ul reactions |
EUR 106.8 |
OneScriptcDNA Synthesis SuperMix |
|
G452 |
ABM |
100 x 20 ul reactions |
EUR 169.2 |
 Fast QCell-Pro One-Step qRT-PCR SuperMix Kit |
|
K5055002 |
Biochain |
200 rxn |
EUR 380 |
 OneScriptPlus cDNA Synthesis SuperMix |
|
G453 |
ABM |
25 x 20 ul reactions |
EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
|
G454 |
ABM |
100 x 20 ul reactions |
EUR 202.8 |
 Green Two-Step qRT-PCR SuperMix |
|
abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 777.6 |
|
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 Green One-Step qRT-PCR SuperMix |
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20-abx09803820ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
|
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 Probe One-Step qRT-PCR SuperMix |
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20-abx09804220ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
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) Two-Step RT-PCR SuperMix (12 kb) |
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abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 727.2 |
|
|
) Two-Step RT-PCR SuperMix (15 kb) |
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abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems |
Abbexa |
50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) |
EUR 910.8 |
|
|
 All-in-One cDNA Synthesis SuperMix |
|
B24403 |
Bimake |
200 ractions |
EUR 547.2 |
|
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
All-in-One cDNA Synthesis SuperMix |
|
B24408 |
Bimake |
1000 ractions |
EUR 1743.6 |
|
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
 (4 kb)) One-Step RT-PCR SuperMix (+dye) (4 kb) |
|
abx098027-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 727.2 |
|
|
 (8 kb)) One-Step RT-PCR SuperMix (+dye) (8 kb) |
|
abx098028-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 794.4 |
|
|
One-Step RT-PCR SuperMix (+dye) (8 kb) |
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abx098029-200rxns20ulSystems |
Abbexa |
200 rxns × 20 ul Systems |
EUR 1028.4 |
|
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 Fly First-Strand cDNA Synthesis SuperMix |
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20-abx09885820ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
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 Green miRNA Two-Step qRT-PCR SuperMix |
|
abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems |
Abbexa |
20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems) |
EUR 1062 |
|
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 miRNA First-Strand cDNA Synthesis SuperMix |
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abx098020-20rxns20ulSystems |
Abbexa |
20 rxns × 20 ul Systems |
EUR 878.4 |
|
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 PCR SuperMix (-dye) (DNA, PCR)) High Fidelity (HiFi) PCR SuperMix (-dye) (DNA, PCR) |
|
20-abx098007 |
Abbexa |
|
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|
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) Green Two-Step qRT-PCR SuperMix (GC Rich) |
|
abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems |
Abbexa |
50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) |
EUR 961.2 |
|
|
) Green One-Step qRT-PCR SuperMix (GC Rich) |
|
20-abx09803920ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
|
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) Probe One-Step qRT-PCR SuperMix (GC Rich) |
|
20-abx09804120ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 400 rxns × 20 ul Systems
|
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 First-Strand cDNA Synthesis SuperMix for PCR |
|
abx098018-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 693.6 |
|
|
 One-Step gDNA Removal and cDNA Synthesis SuperMix |
|
20-abx09885920ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
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) First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
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20-abx09801620ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
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) First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
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20-abx09802120ulSystems |
Abbexa |
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- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
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) One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb) |
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20-abx09802220ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
|
) One-Step gDNA Removal and cDNA Synthesis SuperMix (8 kb) |
|
20-abx09801520ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
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) One-Step gDNA Removal and cDNA Synthesis SuperMix (12 kb) |
|
20-abx09801720ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
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 All-in-One First-Strand cDNA Synthesis SuperMix for PCR |
|
abx098023-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 878.4 |
|
|
 PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA)) High Fidelity (HiFi) PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA) |
|
20-abx098008 |
Abbexa |
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|
 Built in power supply for WSE-1165
Power AC 100-240V |
|
WSE-650-659 |
Atto |
1unit |
EUR 871.2 |
|
Description: Assembl y power suppl y wi t h WSE-1165 such as bui l t -i n |
 electrophoresis power supply |
|
EV245 |
Consort |
ea |
EUR 574.8 |
 Four Source Power Supply |
|
EDK621 |
Bio Basic |
1 UNIT |
EUR 821.08 |
|
|
 Dual Source 150 Power Supply |
|
EDK620 |
Bio Basic |
1 UNIT |
EUR 445.75 |
|
|
 CyanRed Power Box |
|
2305226 |
Atto |
2unit |
EUR 339.6 |
|
Description: i ndepent l y use for CyanRed Epi |
 ENDURO™ 250V Power Supply , 120V |
|
LE0203-120 |
GenDepot |
Ea |
EUR 1923.6 |
 ENDURO™ 250V Power Supply , 230V |
|
LE0203-230 |
GenDepot |
Ea |
EUR 1923.6 |
 ENDURO™ 300V Power Supply , 120V |
|
LE0303-120 |
GenDepot |
Ea |
EUR 1437.6 |
 myVolt™ Mini power supply, 115V |
|
BM0294 |
GenDepot |
Ea |
EUR 368.4 |
 myVolt™ Mini power supply, 230V |
|
BM0295 |
GenDepot |
Ea |
EUR 368.4 |
 myVolt™ Mini Power Supply,115V |
|
LE1007 |
GenDepot |
Ea |
EUR 368.4 |
 myVolt™ Mini Power Supply, 230V |
|
LE1008 |
GenDepot |
Ea |
EUR 368.4 |
 electrophoresis power supply 120 vac |
|
EV245-US |
Consort |
ea |
EUR 574.8 |
 myVolt™ Touch power supply, 115V |
|
BM0296 |
GenDepot |
Ea |
EUR 861.6 |
 myVolt™ Touch power supply, 230V |
|
BM0297 |
GenDepot |
Ea |
EUR 861.6 |
myVolt™ Touch power supply, 115V |
|
LE1009 |
GenDepot |
Ea |
EUR 861.6 |
 myVolt™ Touch power supply, 220V |
|
LE1010 |
GenDepot |
Ea |
EUR 861.6 |
 electrophoresis power supply uk plug |
|
EV245-UK |
Consort |
ea |
EUR 574.8 |
 Power supply for LABeler Lab Printer |
|
L9010-PS |
MTC Bio |
1/pack |
EUR 100.32 |
|
Description: Power supply for Labeler |
 UNIVERSAL POWER SUPPLY FOR AXYGEN MOTOPET |
|
MOTO-SP-PWR |
CORNING |
1/pk |
EUR 73.2 |
|
Description: Handheld Pipettor Repairs; Liquid Handling Device Repairs |
 LABeler Lab Printer w/ 115V power supply |
|
L9010 |
MTC Bio |
1/pack |
EUR 224.76 |
|
Description: LABeler Hand Held Labeler |
 CometAssay 20 Well ES Starter Kit plus NA Power Supply |
|
4252-040-ESK-PS1 |
Biotechne |
1 Kit |
EUR 4597.68 |
 CometAssay 20 Well ES Starter Kit plus EU Power Supply |
|
4252-040-ESK-PS2 |
Biotechne |
1 Kit |
EUR 4597.68 |
 CometAssay 20 Well ES Starter Kit plus SZ Power Supply |
|
4252-040-ESK-PS4 |
Biotechne |
1 Kit |
EUR 4597.68 |
 CometAssay 96 Well ES Starter Kit plus NA Power Supply |
|
4253-096-ESK-PS1 |
Biotechne |
1 Kit |
EUR 4528.8 |
 CometAssay 96 Well ES Starter Kit plus EU Power Supply |
|
4253-096-ESK-PS2 |
Biotechne |
1 Kit |
EUR 4597.68 |
 CometAssay 96 Well ES Starter Kit plus UK Power Supply |
|
4253-096-ESK-PS3 |
Biotechne |
1 Kit |
EUR 4597.68 |
 CometAssay 96 Well ES Starter Kit plus SZ Power Supply |
|
4253-096-ESK-PS4 |
Biotechne |
1 Kit |
EUR 4597.68 |
 CometAssay Electrophoresis Starter Kit plus EU Power Supply |
|
4250-050-ESK-PS2 |
Biotechne |
1 Kit |
EUR 4597.68 |
 CometAssay Electrophoresis Starter Kit plus UK Power Supply |
|
4250-050-ESK-PS3 |
Biotechne |
1 Kit |
EUR 4597.68 |
 CometAssay Electrophoresis Starter Kit plus SZ Power Supply |
|
4250-050-ESK-PS4 |
Biotechne |
1 Kit |
EUR 4597.68 |
 CometAssay 20 Well ES Starter Kit plus AUS Power Supply |
|
4252-040-ESK-PS5 |
Biotechne |
1 Kit |
EUR 4597.68 |
 CometAssay 96 Well ES Starter Kit plus AUS Power Supply |
|
4253-096-ESK-PS5 |
Biotechne |
1 Kit |
EUR 4597.68 |
 Accuris PowerVolt™ High Current, 300V/2000mA Power Supply, 115V |
|
E2302-2000 |
Benchmark Scientific |
1 each |
EUR 1333.8 |
 Accuris PowerVolt™ High Current, 300V/2000mA Power Supply, 230V |
|
E2302-2000-E |
Benchmark Scientific |
1 each |
EUR 1333.8 |
 Animal Detection Probe qPCR Super PreMix |
|
QV114-EN01 |
Vazyme |
400 rxns |
EUR 128 |
Animal Detection Probe qPCR Super PreMix |
|
QV114-EN02 |
Vazyme |
800 rxns |
EUR 230.4 |
 Alkaline phosphatase Power AP |
|
AZ140A |
Bio Basic |
1MG |
EUR 120.55 |
|
|
Alkaline phosphatase Power AP |
|
AZ235A |
Bio Basic |
1MG |
EUR 139.34 |
|
|
 ENDURO™ Mini 300V Power Supply , 110V-220V |
|
LE0304 |
GenDepot |
Ea |
EUR 846 |
 CometAssay 20 Well ES Starter Kit plus Power Supply for UK |
|
4252-040-ESK-PS3 |
Biotechne |
1 Kit |
EUR 4597.68 |
 Power leads for HorizeBLOT-R |
|
2322462 |
Atto |
5unit |
EUR 324 |
The outcomes point out the significance of cell wall modification for quantitative stem rot resistance by responses within the phenylpropanoid metabolism producing distinct lignin varieties on totally different temporal scales.
