Low temperature is a significant abiotic stress that critically limits mangrove productiveness and distribution. Kandelia obovata is probably the most cold-resistance specie in mangrove vegetation, however little is understood concerning the molecular mechanism underlying its resistance to chilly. Osmotin is a key protein related to abiotic and biotic stress response in vegetation however no details about this gene in Ok. obovata was reported.
On this research, a cDNA sequence encoding osmotin, KoOsmotin (GenBank accession no. KP267758), was cloned from mangrove plant Ok. obovata. The KoOsmotin protein was composed of 221 amino acids and confirmed a calculated molecular mass of 24.11 kDa with pI 4.92. The KoOsmotin contained sixteen cysteine residues and an N-terminal sign peptide, which had been frequent signatures to most osmotins and pathogenesis-related 5 proteins. The three-dimensional (3D) mannequin of KoOsmotin, contained one α-helix and eleven β-strands, was fashioned by three attribute domains. Database comparisons of the KoOsmotin confirmed the closest identification (55.75%) with the osmotin 34 from Theobroma cacao. The phylogenetic tree additionally revealed that the KoOsmotin was clustered within the department of osmotin/OLP (osmotin-like protien). The KoOsmotin protein was proved to be localized to each the plasma membrane and cytoplasm by the subcellular localization evaluation. Gene expression confirmed that the KoOsmotin was induced primarily and extremely within the leaves of Ok. obovata, however much less abundantly in stems and roots. The overexpressing of KoOsmotin conferred chilly tolerance in Escherichia coli cells.
CRISPR-Cas9 multiplex genome modifying of the hydroxyproline-O-galactosyltransferase gene household alters arabinogalactan-protein glycosylation and performance in Arabidopsis

Lignin composition and timing of cell wall lignification are concerned in Brassica napus resistance to Sclerotinia sclerotiorum stem rot
Green qPCR SuperMix |
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| abx098031-100l | Abbexa | 100 µl | EUR 675 |
Green qPCR SuperMix |
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| 20-abx098031 | Abbexa |
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Green qPCR SuperMix |
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| abx098031-200l | Abbexa | 200 µl | EUR 912.5 |
Probe qPCR SuperMix |
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| 20-abx098040 | Abbexa |
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Green qPCR SuperMix UDG |
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| abx098032-100l | Abbexa | 100 µl | EUR 687.5 |
Green qPCR SuperMix UDG |
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| 20-abx098032 | Abbexa |
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Green qPCR SuperMix UDG |
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| abx098032-200l | Abbexa | 200 µl | EUR 912.5 |
Top Green qPCR SuperMix |
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| abx098033-100l | Abbexa | 100 µl | EUR 262.5 |
Top Green qPCR SuperMix |
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| 20-abx098033 | Abbexa |
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Top Green qPCR SuperMix |
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| abx098033-200l | Abbexa | 200 µl | EUR 487.5 |
Tip Green qPCR SuperMix |
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| abx098034-100l | Abbexa | 100 µl | EUR 262.5 |
Tip Green qPCR SuperMix |
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| 20-abx098034 | Abbexa |
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Tip Green qPCR SuperMix |
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| abx098034-200l | Abbexa | 200 µl | EUR 437.5 |
Library Quantification qPCR SuperMix |
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| abx098896-100l | Abbexa | 100 µl | EUR 700 |
Library Quantification qPCR SuperMix |
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| abx098896-200l | Abbexa | 200 µl | EUR 962.5 |
Library Quantification qPCR SuperMix |
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| 20-abx098896 | Abbexa |
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EnTurbo™ probe qPCR SuperMix |
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| EQ017 | ELK Biotech | 5mL | EUR 240 |
EnTurbo™ probe qPCR SuperMix |
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| EQ017-25mL | ELK Biotech | 25mL | EUR 240 |
EnTurbo™ probe qPCR SuperMix |
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| EQ017-5mL | ELK Biotech | 5mL | EUR 78 |
Top Green qPCR SuperMix (+Dye II) |
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| abx098890-100l | Abbexa | 100 µl | EUR 262.5 |
Top Green qPCR SuperMix (+Dye II) |
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| 20-abx098890 | Abbexa |
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Top Green qPCR SuperMix (+Dye II) |
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| abx098890-200l | Abbexa | 200 µl | EUR 487.5 |
Tip Green qPCR SuperMix (+Dye II) |
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| abx098891-100l | Abbexa | 100 µl | EUR 262.5 |
Tip Green qPCR SuperMix (+Dye II) |
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| 20-abx098891 | Abbexa |
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Tip Green qPCR SuperMix (+Dye II) |
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| abx098891-200l | Abbexa | 200 µl | EUR 437.5 |
EnTurbo™ SYBR Color qPCR SuperMix |
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| EQ036 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix |
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| EQ036-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix |
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| EQ036-5mL | ELK Biotech | 5mL | EUR 80 |
cDNA Synthesis SuperMix for qPCR |
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| abx098019-100l | Abbexa | 100 µl | EUR 825 |
cDNA Synthesis SuperMix for qPCR |
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| 20-abx09801920ulSystems | Abbexa |
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cDNA Synthesis SuperMix for qPCR |
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| abx098019-1ml | Abbexa | 1 ml | EUR 2387.5 |
cDNA Synthesis SuperMix for qPCR |
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| abx098019-200l | Abbexa | 200 µl | EUR 1000 |
HyperScript™ RT SuperMix for qPCR |
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| K1074-100 | ApexBio | 100 rxn (20 uL/rxn) | EUR 296 |
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Description: Reverse Transcription & PCR|Reverse Transcription |
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HyperScript™ RT SuperMix for qPCR |
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| K1074-50 | ApexBio | 50 rxn (20 uL/rxn) | EUR 166.4 |
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Description: Reverse Transcription & PCR|Reverse Transcription |
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HiScript II Q RT SuperMix for qPCR |
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| R222-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 145 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
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| EQ035 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
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| EQ035-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
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| EQ035-5mL | ELK Biotech | 5mL | EUR 80 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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| abx098024-100l | Abbexa | 100 µl | EUR 1025 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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| abx098024-1ml | Abbexa | 1 ml | Ask for price |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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| abx098024-200l | Abbexa | 200 µl | Ask for price |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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| abx098024-50rxns20ulSystems | Abbexa | 50 rxns × 20 ul Systems | EUR 861.6 |
HiScript II Q Select RT SuperMix for qPCR |
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| R232-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 145 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
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| R423-00-10rxns | Vazyme | 10 rxns | EUR 1.09 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
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| R423-01 | Vazyme | 100rxns(20µl/rxn) | EUR 326 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
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| R423-01-100rxns | Vazyme | 100 rxns | EUR 337.36 |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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| abx098860-100l | Abbexa | 100 µl | EUR 1012.5 |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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| abx098860-1ml | Abbexa | 1 ml | Ask for price |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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| abx098860-200l | Abbexa | 200 µl | Ask for price |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
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| R323-00-10rxns | Vazyme | 10 rxns | EUR 1.09 |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
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| R323-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 217.5 |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
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| R323-01-100rxns | Vazyme | 100 rxns | EUR 225.22 |
EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed) |
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| EQ033 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed) |
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| EQ033-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed) |
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| EQ033-5mL | ELK Biotech | 5mL | EUR 80 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
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| EQ034 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
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| EQ034-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
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| EQ034-5mL | ELK Biotech | 5mL | EUR 80 |
HiScript II Q RT SuperMix for qPCR (+gDNA wiper) |
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| R223-01 | Vazyme | 100rxns(20µl/rxn) | EUR 159.5 |
HyperScript™ RT SuperMix for qPCR (with gDNA wiper) |
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| K1174-100 | ApexBio | 100 rxns | EUR 320 |
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Description: Reverse Transcription & PCR|Reverse Transcription |
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HyperScript™ RT SuperMix for qPCR (with gDNA wiper) |
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| K1174-50 | ApexBio | 50 rxns | EUR 192 |
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Description: Reverse Transcription & PCR|Reverse Transcription |
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HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper) |
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| R233-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 159.5 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
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| R333-00-10rxns | Vazyme | 10 rxns | EUR 1.09 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
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| R333-01 | Vazyme | 100 rxns (21 μl/rxn) | EUR 217.5 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
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| R333-01-100rxns | Vazyme | 100 rxns | EUR 225.22 |
Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus) |
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| 11141ES10 | Yeasen Biotechnology | 10T | EUR 25 |
Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus) |
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| 11141ES60 | Yeasen Biotechnology | 100T | EUR 230 |
PCR SuperMix |
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| 20-abx098887 | Abbexa |
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2X PCR SuperMix |
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| MB200-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 11 |
2X PCR SuperMix |
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| MBS402025-100Reactions | MyBiosource | 100Reactions | EUR 175 |
2X PCR SuperMix |
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| MBS402025-4Reactions | MyBiosource | 4Reactions | EUR 160 |
2X PCR SuperMix |
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| MB200-P100S | Bio-Helix | 4 Reactions | Ask for price |
Eva-QPCR-SuperMix-Kit |
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| K5052200 | Biochain | 200 reactions | EUR 147.7 |
Eva-QPCR-SuperMix-Kit |
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| K5052400 | Biochain | 400 reactions | EUR 247.8 |
PCR SuperMix (-dye) |
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| abx098002-100l | Abbexa | 100 µl | EUR 237.5 |
PCR SuperMix (-dye) |
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| 20-abx098002 | Abbexa |
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PCR SuperMix (-dye) |
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| abx098002-200l | Abbexa | 200 µl | EUR 337.5 |
PCR SuperMix (+dye) |
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| abx098003-100l | Abbexa | 100 µl | EUR 237.5 |
PCR SuperMix (+dye) |
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| 20-abx098003 | Abbexa |
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PCR SuperMix (+dye) |
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| abx098003-200l | Abbexa | 200 µl | EUR 337.5 |
Plus PCR SuperMix |
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| abx098888-100l | Abbexa | 100 µl | EUR 300 |
Plus PCR SuperMix |
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| abx098888-200l | Abbexa | 200 µl | EUR 612.5 |
Plus PCR SuperMix |
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| 20-abx098888 | Abbexa |
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Evo? cDNA Supermix |
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| M1168-100 | Biovision | each | EUR 457.2 |
Evo? cDNA Supermix |
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| M1168-25 | Biovision | each | EUR 320.4 |
Novo? cDNA Supermix |
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| M1169-100 | Biovision | each | EUR 529.2 |
Novo? cDNA Supermix |
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| M1169-25 | Biovision | each | EUR 346.8 |
HotStar PCR Supermix |
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| MB201-0100 | GeneDireX | 100 Reactions | Ask for price |
Probe Direct SuperMix |
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| abx461057-1096tests | Abbexa | 10 × 96 tests | Ask for price |
Probe Direct SuperMix |
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| abx461057-596tests | Abbexa | 5 × 96 tests | EUR 2800 |
Probe Direct SuperMix |
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| abx461057-96tests | Abbexa | 96 tests | EUR 812.5 |
cDNA Synthesis SuperMix |
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| abx098014-100l | Abbexa | 100 µl | EUR 725 |
cDNA Synthesis SuperMix |
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| 20-abx09801420ulSystems | Abbexa |
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cDNA Synthesis SuperMix |
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| abx098014-1ml | Abbexa | 1 ml | Ask for price |
cDNA Synthesis SuperMix |
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| abx098014-200l | Abbexa | 200 µl | EUR 812.5 |
T PCR SuperMix (-dye) |
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| abx098005-100l | Abbexa | 100 µl | EUR 262.5 |
T PCR SuperMix (-dye) |
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| abx098005-200l | Abbexa | 200 µl | EUR 462.5 |
T PCR SuperMix (-dye) |
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| 20-abx098005 | Abbexa |
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T PCR SuperMix (+dye) |
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| abx098006-100l | Abbexa | 100 µl | EUR 262.5 |
T PCR SuperMix (+dye) |
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| abx098006-200l | Abbexa | 200 µl | EUR 462.5 |
T PCR SuperMix (+dye) |
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| 20-abx098006 | Abbexa |
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AmpHS 2X PCR SuperMix |
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| MB203-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 11 |
AmpHS 2X PCR SuperMix |
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| MB203-P040 | Bio-Helix | 40 Reactions | Ask for price |
EasyPfu PCR SuperMix (-dye) |
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| 20-abx098009 | Abbexa |
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FastPfu PCR SuperMix (-dye) |
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| abx098010-100l | Abbexa | 100 µl | EUR 300 |
FastPfu PCR SuperMix (-dye) |
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| abx098010-200l | Abbexa | 200 µl | EUR 575 |
FastPfu PCR SuperMix (-dye) |
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| 20-abx098010 | Abbexa |
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Library Amplification SuperMix |
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| abx098894-100l | Abbexa | 100 µl | EUR 737.5 |
Library Amplification SuperMix |
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| abx098894-200l | Abbexa | 200 µl | EUR 1137.5 |
Library Amplification SuperMix |
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| 20-abx098894 | Abbexa |
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OneScriptcDNA Synthesis SuperMix |
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| G451 | ABM | 25 x 20 ul reactions | EUR 106.8 |
OneScriptcDNA Synthesis SuperMix |
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| G452 | ABM | 100 x 20 ul reactions | EUR 169.2 |
PCR SuperMix for PAGE (+dye) |
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| abx098004-100l | Abbexa | 100 µl | EUR 237.5 |
PCR SuperMix for PAGE (+dye) |
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| 20-abx098004 | Abbexa |
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PCR SuperMix for PAGE (+dye) |
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| abx098004-200l | Abbexa | 200 µl | EUR 337.5 |
OneScriptPlus cDNA Synthesis SuperMix |
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| G453 | ABM | 25 x 20 ul reactions | EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
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| G454 | ABM | 100 x 20 ul reactions | EUR 202.8 |
OmniPCR Supermix w Fluorescent dye |
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| MBA01-0100 | Bio-Helix | 100 rxns (2.5ml) | EUR 16 |
OmniPCR Supermix w Fluorescent dye |
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| MBA01-0100S | Bio-Helix | 4 Reactions | Ask for price |
EnTurbo™ SYBR Green PCR SuperMix |
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| EQ001 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix |
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| EQ001-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix |
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| EQ001-5mL | ELK Biotech | 5mL | EUR 80 |
Green Two-Step qRT-PCR SuperMix |
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| abx098035-100l | Abbexa | 100 µl | EUR 925 |
Green Two-Step qRT-PCR SuperMix |
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| abx098035-1ml | Abbexa | 1 ml | Ask for price |
Green Two-Step qRT-PCR SuperMix |
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| abx098035-200l | Abbexa | 200 µl | Ask for price |
Green Two-Step qRT-PCR SuperMix |
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| abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems | Abbexa | 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) | EUR 777.6 |
Green One-Step qRT-PCR SuperMix |
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| abx098038-100l | Abbexa | 100 µl | EUR 737.5 |
Green One-Step qRT-PCR SuperMix |
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| 20-abx09803820ulSystems | Abbexa |
|
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Green One-Step qRT-PCR SuperMix |
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| abx098038-1ml | Abbexa | 1 ml | Ask for price |
Green One-Step qRT-PCR SuperMix |
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| abx098038-200l | Abbexa | 200 µl | EUR 1050 |
Probe One-Step qRT-PCR SuperMix |
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| abx098042-100l | Abbexa | 100 µl | EUR 850 |
Probe One-Step qRT-PCR SuperMix |
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| 20-abx09804220ulSystems | Abbexa |
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Probe One-Step qRT-PCR SuperMix |
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| abx098042-1ml | Abbexa | 1 ml | Ask for price |
Probe One-Step qRT-PCR SuperMix |
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| abx098042-200l | Abbexa | 200 µl | EUR 1400 |
AmpLong 2X PCR SuperMix (15-30 sec/kb; ≤20kb, 1X) |
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| MB204-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 13 |
Two-Step RT-PCR SuperMix (12 kb) |
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| abx098025-100l | Abbexa | 100 µl | EUR 887.5 |
Two-Step RT-PCR SuperMix (12 kb) |
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| abx098025-1ml | Abbexa | 1 ml | Ask for price |
Two-Step RT-PCR SuperMix (12 kb) |
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| abx098025-200l | Abbexa | 200 µl | Ask for price |
Two-Step RT-PCR SuperMix (12 kb) |
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| abx098025-50rxns20ulRTSystems80rxns50ulPCRSystems | Abbexa | 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) | EUR 727.2 |
Two-Step RT-PCR SuperMix (15 kb) |
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| abx098026-100l | Abbexa | 100 µl | EUR 1087.5 |
Two-Step RT-PCR SuperMix (15 kb) |
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| abx098026-1ml | Abbexa | 1 ml | Ask for price |
Two-Step RT-PCR SuperMix (15 kb) |
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| abx098026-200l | Abbexa | 200 µl | Ask for price |
Two-Step RT-PCR SuperMix (15 kb) |
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| abx098026-50rxns20ulRTSystems80rxns50ulPCRSystems | Abbexa | 50 rxns × 20 ul (RT Systems) / 80 rxns × 50 ul (PCR Systems) | EUR 910.8 |
OmniPCR HS Supermix w Fluorescent dye |
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| MBA03-0100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 16 |
All-in-One cDNA Synthesis SuperMix |
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| B24403 | Bimake | 200 ractions | EUR 547.2 |
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Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
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All-in-One cDNA Synthesis SuperMix |
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| B24408 | Bimake | 1000 ractions | EUR 1743.6 |
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Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription. |
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AmpFAST 2X PCR SuperMix (5-30 sec/kb; ≤6kb, 1X) |
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| MB202-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 11 |
OmniPCR FAST Supermix w Fluorescent dye |
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| MBA02-0100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 16 |
OmniPCR Long Supermix w Fluorescent dye |
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| MBA04-0100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 16 |
OnePCR Ultra Supermix w Fluorescent Dye |
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| MBS402026-100Reactions | MyBiosource | 100Reactions | EUR 180 |
OnePCR Ultra Supermix w Fluorescent Dye |
|||
| MBS402026-4Reactions | MyBiosource | 4Reactions | EUR 160 |
Fly First-Strand cDNA Synthesis SuperMix |
|||
| abx098858-100l | Abbexa | 100 µl | EUR 850 |
Fly First-Strand cDNA Synthesis SuperMix |
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| 20-abx09885820ulSystems | Abbexa |
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Fly First-Strand cDNA Synthesis SuperMix |
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| abx098858-1ml | Abbexa | 1 ml | Ask for price |
Fly First-Strand cDNA Synthesis SuperMix |
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| abx098858-200l | Abbexa | 200 µl | EUR 1037.5 |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
|||
| abx098027-100l | Abbexa | 100 µl | EUR 875 |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
|||
| abx098027-1ml | Abbexa | 1 ml | Ask for price |
One-Step RT-PCR SuperMix (+dye) (4 kb) |
|||
| abx098027-200l | Abbexa | 200 µl | Ask for price |
