Research Progress on the Anti-Cancer Molecular Mechanisms of Huaier

The Brassica coenospeceis have treasure troves of genes that could be beneficial if introgressed into cultivated Brassicas to combat the current conditions of climate change. Introducing genetic variability through plant speciation with polyploidization is well documented, where ploidy augmentation of inter-generic allohexaploids using somatic hybridization has significantly contributed to genetic base broadening. Sinapis alba is a member of the Brassicaceae family that possesses valuable genes, including genes conferring resistance to Sclerotinia sclerotiorum, Alternaria brassicae, pod shattering, heat, and drought stress.

This work aimed to synthesize stable allohexaploid (AABBSS) Brassica while incorporating the yellow-seed trait and resistance to S. sclerotiorum stem rot. The two fertile and stable allohexaploids were developed by polyethylene glycol mediated protoplast fusions between Brassica juncea and S. alba (SS) and named as JS1 and JS2. These symmetric hybrids were validated using morphological and molecular cytology techniques and were found to be stable over consecutive generations. The complete chromosome constitution of the three genomes was determined through genomic in situ hybridization of mitotic cells probed with S. alba genomic DNA labeled with fluorescein isothiocyanate.

These two allohexaploids showed 24 hybridization signals demonstrating the presence of complete diploid chromosomes from S. alba and 36 chromosomes from B. juncea. The meiotic pollen mother cell showed 30 bivalent sets of all the 60 chromosomes and none of univalent or trivalent observed during meiosis.

Helical growth is an economical way for plant to obtain resources. The classic microtubule-microfibril alignment model of Arabidopsis helical growth involves restriction of the appropriate orientation of cellulose microfibrils appropriately in the cell walls. However, the molecular mechanism underlying tomato helical growth remains unknown. Here, we identified a spontaneous tomato helical (hel) mutant with right-handed helical cotyledons and petals but left-handed helical stems and true leaves.

Genetic analysis revealed that the hel phenotype was controlled by a single recessive gene. Using map-based cloning, we cloned the HEL gene, which encodes a cellulose interacting protein homologous to CSI1 of Arabidopsis. We identified a 27 bp fragment replacement that generated a premature stop codon. Transgenic experiments showed that the helical growth phenotype could be restored by the allele of this gene from wild-type Pyriforme. In contrast, the knockout mutation of HEL in Pyriforme via CRISPR/Cas9 resulted in helical growth. These findings shed light on the molecular control of the helical growth of tomato.

Many features that are appropriate for an ideal tissue engineered biomaterial are found in plant tissues. Hierarchically organized Bambusa vulgaris exhibits structural similarities to native bone, but the degradation of cellulose that is the main component of the plant cell wall is a challenge. In this study, Bamboo stem was subjected to decellularization followed by a chemical oxidation process (treated with sodium periodate) to enhance biocompatibility and biodegradation.

Green qPCR SuperMix
abx098031-200l	Abbexa	200 µl	EUR 912.5

Green qPCR SuperMix UDG
abx098032-100l	Abbexa	100 µl	EUR 687.5

Green qPCR SuperMix UDG
20-abx098032	Abbexa	Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml

Green qPCR SuperMix UDG
abx098032-200l	Abbexa	200 µl	EUR 912.5

Tip Green qPCR SuperMix
abx098034-100l	Abbexa	100 µl	EUR 262.5

Tip Green qPCR SuperMix
20-abx098034	Abbexa	Ask for price Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml 25 ml

Tip Green qPCR SuperMix
abx098034-200l	Abbexa	200 µl	EUR 437.5

Tip Green qPCR SuperMix (+Dye II)
abx098891-100l	Abbexa	100 µl	EUR 262.5

Tip Green qPCR SuperMix (+Dye II)
20-abx098891	Abbexa	Ask for price Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml 25 ml

Tip Green qPCR SuperMix (+Dye II)
abx098891-200l	Abbexa	200 µl	EUR 437.5

Probe qPCR SuperMix
20-abx098040	Abbexa	Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml

EnTurbo™ SYBR Green PCR SuperMix
EQ001	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix
EQ001-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix
EQ001-5mL	ELK Biotech	5mL	EUR 80

Library Quantification qPCR SuperMix
abx098896-100l	Abbexa	100 µl	EUR 700

Library Quantification qPCR SuperMix
abx098896-200l	Abbexa	200 µl	EUR 962.5

Library Quantification qPCR SuperMix
20-abx098896	Abbexa	Ask for price Ask for price	1 ml 5 ml

Green Two-Step qRT-PCR SuperMix
abx098035-100l	Abbexa	100 µl	EUR 925

Green Two-Step qRT-PCR SuperMix
abx098035-1ml	Abbexa	1 ml	Ask for price

Green Two-Step qRT-PCR SuperMix
abx098035-200l	Abbexa	200 µl	Ask for price

Green Two-Step qRT-PCR SuperMix
abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems	Abbexa	50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)	EUR 777.6

Green One-Step qRT-PCR SuperMix
abx098038-100l	Abbexa	100 µl	EUR 737.5

Green One-Step qRT-PCR SuperMix
20-abx09803820ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Green One-Step qRT-PCR SuperMix
abx098038-1ml	Abbexa	1 ml	Ask for price

Green One-Step qRT-PCR SuperMix
abx098038-200l	Abbexa	200 µl	EUR 1050

EnTurbo™ probe qPCR SuperMix
EQ017	ELK Biotech	5mL	EUR 240

EnTurbo™ probe qPCR SuperMix
EQ017-25mL	ELK Biotech	25mL	EUR 240

EnTurbo™ probe qPCR SuperMix
EQ017-5mL	ELK Biotech	5mL	EUR 78

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-100l	Abbexa	100 µl	EUR 1212.5

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-1ml	Abbexa	1 ml	Ask for price

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-200l	Abbexa	200 µl	Ask for price

Green miRNA Two-Step qRT-PCR SuperMix
abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems	Abbexa	20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems)	EUR 1062

EnTurbo™ SYBR Color qPCR SuperMix
EQ036	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix
EQ036-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix
EQ036-5mL	ELK Biotech	5mL	EUR 80

cDNA Synthesis SuperMix for qPCR
abx098019-100l	Abbexa	100 µl	EUR 825

cDNA Synthesis SuperMix for qPCR
20-abx09801920ulSystems	Abbexa	Ask for price Ask for price	50 rxns × 20 ul Systems 100 rxns ×20 ul Systems

cDNA Synthesis SuperMix for qPCR
abx098019-1ml	Abbexa	1 ml	EUR 2387.5

cDNA Synthesis SuperMix for qPCR
abx098019-200l	Abbexa	200 µl	EUR 1000

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-100l	Abbexa	100 µl	EUR 1125

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-1ml	Abbexa	1 ml	Ask for price

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-200l	Abbexa	200 µl	Ask for price

Green Two-Step qRT-PCR SuperMix (GC Rich)
abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems	Abbexa	50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems)	EUR 961.2

Green One-Step qRT-PCR SuperMix (GC Rich)
abx098039-100l	Abbexa	100 µl	EUR 450

Green One-Step qRT-PCR SuperMix (GC Rich)
20-abx09803920ulSystems	Abbexa	Ask for price Ask for price	100 rxns × 20 ul Systems 400 rxns × 20 ul Systems

Green One-Step qRT-PCR SuperMix (GC Rich)
abx098039-1ml	Abbexa	1 ml	Ask for price

Green One-Step qRT-PCR SuperMix (GC Rich)
abx098039-200l	Abbexa	200 µl	EUR 1000

HyperScript™ RT SuperMix for qPCR
K1074-100	ApexBio	100 rxn (20 uL/rxn)	EUR 296
Description: Reverse Transcription & PCR|Reverse Transcription

HyperScript™ RT SuperMix for qPCR
K1074-50	ApexBio	50 rxn (20 uL/rxn)	EUR 166.4
Description: Reverse Transcription & PCR|Reverse Transcription

HiScript II Q RT SuperMix for qPCR
R222-01	Vazyme	100 rxn (20 μl/rxn)	EUR 145

EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed)
EQ014	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed)
EQ014-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed)
EQ014-5mL	ELK Biotech	5mL	EUR 80

EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed)
EQ013	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed)
EQ013-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed)
EQ013-5mL	ELK Biotech	5mL	EUR 80

EnTurbo™ SYBR Color qPCR SuperMix (Without ROX)
EQ035	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (Without ROX)
EQ035-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (Without ROX)
EQ035-5mL	ELK Biotech	5mL	EUR 80

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-100l	Abbexa	100 µl	EUR 1025

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-1ml	Abbexa	1 ml	Ask for price

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-200l	Abbexa	200 µl	Ask for price

cDNA Synthesis SuperMix for qPCR (GC-rich)
abx098024-50rxns20ulSystems	Abbexa	50 rxns × 20 ul Systems	EUR 861.6

HiScript II Q Select RT SuperMix for qPCR
R232-01	Vazyme	100 rxn (20 μl/rxn)	EUR 145

HiScript IV RT SuperMix for qPCR (+gDNA wiper)
R423-00-10rxns	Vazyme	10 rxns	EUR 1.09

HiScript IV RT SuperMix for qPCR (+gDNA wiper)
R423-01	Vazyme	100rxns(20µl/rxn)	EUR 326

HiScript IV RT SuperMix for qPCR (+gDNA wiper)
R423-01-100rxns	Vazyme	100 rxns	EUR 337.36

Uni Cell to cDNA Synthesis SuperMix for qPCR
abx098860-100l	Abbexa	100 µl	EUR 1012.5

Uni Cell to cDNA Synthesis SuperMix for qPCR
abx098860-1ml	Abbexa	1 ml	Ask for price

Uni Cell to cDNA Synthesis SuperMix for qPCR
abx098860-200l	Abbexa	200 µl	Ask for price

HiScript III RT SuperMix for qPCR (+gDNA wiper)
R323-00-10rxns	Vazyme	10 rxns	EUR 1.09

HiScript III RT SuperMix for qPCR (+gDNA wiper)
R323-01	Vazyme	100 rxn (20 μl/rxn)	EUR 217.5

HiScript III RT SuperMix for qPCR (+gDNA wiper)
R323-01-100rxns	Vazyme	100 rxns	EUR 225.22

EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed)
EQ033	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed)
EQ033-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed)
EQ033-5mL	ELK Biotech	5mL	EUR 80

EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed)
EQ034	ELK Biotech	5mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed)
EQ034-25mL	ELK Biotech	25mL	EUR 340

EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed)
EQ034-5mL	ELK Biotech	5mL	EUR 80

HiScript II Q RT SuperMix for qPCR (+gDNA wiper)
R223-01	Vazyme	100rxns(20µl/rxn)	EUR 159.5

HyperScript™ RT SuperMix for qPCR (with gDNA wiper)
K1174-100	ApexBio	100 rxns	EUR 320
Description: Reverse Transcription & PCR|Reverse Transcription

HyperScript™ RT SuperMix for qPCR (with gDNA wiper)
K1174-50	ApexBio	50 rxns	EUR 192
Description: Reverse Transcription & PCR|Reverse Transcription

HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper)
R233-01	Vazyme	100 rxn (20 μl/rxn)	EUR 159.5

HiScript III All-in-one RT SuperMix Perfect for qPCR
R333-00-10rxns	Vazyme	10 rxns	EUR 1.09

HiScript III All-in-one RT SuperMix Perfect for qPCR
R333-01	Vazyme	100 rxns (21 μl/rxn)	EUR 217.5

HiScript III All-in-one RT SuperMix Perfect for qPCR
R333-01-100rxns	Vazyme	100 rxns	EUR 225.22

Malachite Green Phosphate Assay kit
65-POMG-25H	Gentaur Genprice	2500 tests	EUR 333

Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus)
11141ES10	Yeasen Biotechnology	10T	EUR 25

Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus)
11141ES60	Yeasen Biotechnology	100T	EUR 230

PCR SuperMix
20-abx098887	Abbexa	Ask for price Ask for price	1 ml 5 ml

2X PCR SuperMix
MB200-P100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 11

2X PCR SuperMix
MBS402025-100Reactions	MyBiosource	100Reactions	EUR 175

2X PCR SuperMix
MBS402025-4Reactions	MyBiosource	4Reactions	EUR 160

PCR SuperMix (-dye)
abx098002-100l	Abbexa	100 µl	EUR 237.5

PCR SuperMix (-dye)
20-abx098002	Abbexa	Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml

PCR SuperMix (-dye)
abx098002-200l	Abbexa	200 µl	EUR 337.5

PCR SuperMix (+dye)
abx098003-100l	Abbexa	100 µl	EUR 237.5

PCR SuperMix (+dye)
20-abx098003	Abbexa	Ask for price Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml 480 ml

PCR SuperMix (+dye)
abx098003-200l	Abbexa	200 µl	EUR 337.5

Plus PCR SuperMix
abx098888-100l	Abbexa	100 µl	EUR 300

Plus PCR SuperMix
abx098888-200l	Abbexa	200 µl	EUR 612.5

Plus PCR SuperMix
20-abx098888	Abbexa	Ask for price Ask for price	1 ml 5 ml

Evo? cDNA Supermix
M1168-100	Biovision	each	EUR 457.2

Evo? cDNA Supermix
M1168-25	Biovision	each	EUR 320.4

Novo? cDNA Supermix
M1169-100	Biovision	each	EUR 529.2

Novo? cDNA Supermix
M1169-25	Biovision	each	EUR 346.8

Probe Direct SuperMix
abx461057-1096tests	Abbexa	10 × 96 tests	Ask for price

Probe Direct SuperMix
abx461057-596tests	Abbexa	5 × 96 tests	EUR 2800

Probe Direct SuperMix
abx461057-96tests	Abbexa	96 tests	EUR 812.5

cDNA Synthesis SuperMix
abx098014-100l	Abbexa	100 µl	EUR 725

cDNA Synthesis SuperMix
20-abx09801420ulSystems	Abbexa	Ask for price Ask for price	50 rxns × 20 ul Systems 100 rxns × 20 ul Systems

cDNA Synthesis SuperMix
abx098014-1ml	Abbexa	1 ml	Ask for price

cDNA Synthesis SuperMix
abx098014-200l	Abbexa	200 µl	EUR 812.5

T PCR SuperMix (-dye)
abx098005-100l	Abbexa	100 µl	EUR 262.5

T PCR SuperMix (-dye)
abx098005-200l	Abbexa	200 µl	EUR 462.5

T PCR SuperMix (-dye)
20-abx098005	Abbexa	Ask for price Ask for price	1 ml 5 ml

T PCR SuperMix (+dye)
abx098006-100l	Abbexa	100 µl	EUR 262.5

T PCR SuperMix (+dye)
abx098006-200l	Abbexa	200 µl	EUR 462.5

T PCR SuperMix (+dye)
20-abx098006	Abbexa	Ask for price Ask for price	1 ml 5 ml

AmpHS 2X PCR SuperMix
MB203-P100	Bio-Helix	100 rxns2 x 1.25 mL	EUR 11

2×SYBR Green qPCR Mix
E37F021-10x5mL	EnoGene	10×5mL	EUR 1360
Description: DsDNA, ssDNA, RNA quantitative detection kit

2×SYBR Green qPCR Mix
E37F021-5mL	EnoGene	5mL	EUR 227
Description: Fluorescent quantitative PCR kits

2×SYBR Green qPCR Mix
MBS8579840-200Tests	MyBiosource	200Tests	EUR 285

2×SYBR Green qPCR Mix
MBS8579840-50Tests	MyBiosource	50Tests	EUR 180

2×SYBR Green qPCR Mix
MBS8579840-5x200Tests	MyBiosource	5x200Tests	EUR 1230

2×SYBR Green qPCR Mix
MBS8579840-5x50Tests	MyBiosource	5x50Tests	EUR 770

EasyPfu PCR SuperMix (-dye)
20-abx098009	Abbexa	Ask for price Ask for price	1 ml 5 ml

FastPfu PCR SuperMix (-dye)
abx098010-100l	Abbexa	100 µl	EUR 300

FastPfu PCR SuperMix (-dye)
abx098010-200l	Abbexa	200 µl	EUR 575

FastPfu PCR SuperMix (-dye)
20-abx098010	Abbexa	Ask for price Ask for price	1 ml 5 ml

Library Amplification SuperMix
abx098894-100l	Abbexa	100 µl	EUR 737.5

Library Amplification SuperMix
abx098894-200l	Abbexa	200 µl	EUR 1137.5

Library Amplification SuperMix
20-abx098894	Abbexa	Ask for price Ask for price	1 ml 5 ml

miRNA SYBR Green qPCR Mix
4308A	Unibiotest	100 Tests*20μL	EUR 80

miRNA SYBR Green qPCR Mix
4308B	Unibiotest	200 Tests*20μL	EUR 150

miRNA SYBR Green qPCR Mix
4308C	Unibiotest	500 Tests*20μL	EUR 300

SYBR Green qPCR Master Mix
HY-K0501	MedChemExpress	5 mL (500 rxns)	EUR 315.6

OneScriptcDNA Synthesis SuperMix
G451	ABM	25 x 20 ul reactions	EUR 106.8

OneScriptcDNA Synthesis SuperMix
G452	ABM	100 x 20 ul reactions	EUR 169.2

EnoGene Green qPCR Master Mix
E31M001	EnoGene	500 reaction	EUR 75

PCR SuperMix for PAGE (+dye)
abx098004-100l	Abbexa	100 µl	EUR 237.5

PCR SuperMix for PAGE (+dye)
20-abx098004	Abbexa	Ask for price Ask for price Ask for price	1 ml 5 ml 15 ml

PCR SuperMix for PAGE (+dye)
abx098004-200l	Abbexa	200 µl	EUR 337.5

The crystallinity of oxidised plant scaffolds was reduced, resulting in lower mechanical strength. In contrast, hydrophilicity was enhanced in those scaffolds.