Huaier (Trametes robiniophila Murr), a Chinese traditional herb of medicine, has demonstrated promising curative effects in clinical treatment for various tumors. There are documented experiments showing the biological functions of Huaier with its antineoplastic molecular mechanisms: restraining proliferation and metastasis, arresting cell cycle, inducing apoptosis, pyrosis, and autophagy, anti-intratumoral angiogenesis, attenuating characteristics of tumor stem-like cells, interfering with the function of the tumor-related immune system, reversing drug resistance, and enhancing the sensitivity to chemotherapeutic drugs, etc.
In addition, studies suggest that non-coding RNA (ncRNA) acts a pivotal part in cancer occurrence and development, and demonstrates that Huaier adjusts the performance of certain lncRNA (long non-coding RNA) and proceeds to affect the microRNA and its target genes, rendering an anti-tumor effect. Huaier also modulates the expression of lncRNA to attenuate the activity of ncRNA-sponged microRNA and then inhibits the expression of downstream target genes. We summarize and illustrate the experimentally confirmed anti-cancer molecular mechanisms of Huaier, to inspire new ideas for researchers in relevant fields.
Intensive agriculture activities, industrialization and growing numbers of wastewater treatment plants along river banks collectively contribute to the elevated levels of neurotoxic pollutants in natural water reservoirs across Europe. We established an in vitro bioassay based upon neural stem cells isolated from the subventricular zone of the postnatal mouse to evaluate the neurotoxic potential of raw wastewater, treated sewage effluent, groundwater and drinking water. The toxic potential of water samples was evaluated employing viability, proliferation, differentiation and migration assays.
We found that raw wastewater could reduce the viability and proliferation of neural stem cells, and decreased the neuronal and astrocyte differentiation, neuronal neurite growth, astrocyte growth and cell migration. Treated sewage water also showed inhibitory effects on cell proliferation and migration. Our results indicated that relatively high concentrations of nitrogenous substances, pesticides, mercuric compounds, bisphenol-A, and phthalates, along with some other pollutants in raw wastewater and treated sewage water, might be the reason for the neuroinhibitory effects of these water samples.
Development of a Yellow-Seeded Stable Allohexaploid Brassica Through Inter-Generic Somatic Hybridization With a High Degree of Fertility and Resistance to Sclerotinia sclerotiorum
The Brassica coenospeceis have treasure troves of genes that could be beneficial if introgressed into cultivated Brassicas to combat the current conditions of climate change. Introducing genetic variability through plant speciation with polyploidization is well documented, where ploidy augmentation of inter-generic allohexaploids using somatic hybridization has significantly contributed to genetic base broadening. Sinapis alba is a member of the Brassicaceae family that possesses valuable genes, including genes conferring resistance to Sclerotinia sclerotiorum, Alternaria brassicae, pod shattering, heat, and drought stress.
This work aimed to synthesize stable allohexaploid (AABBSS) Brassica while incorporating the yellow-seed trait and resistance to S. sclerotiorum stem rot. The two fertile and stable allohexaploids were developed by polyethylene glycol mediated protoplast fusions between Brassica juncea and S. alba (SS) and named as JS1 and JS2. These symmetric hybrids were validated using morphological and molecular cytology techniques and were found to be stable over consecutive generations. The complete chromosome constitution of the three genomes was determined through genomic in situ hybridization of mitotic cells probed with S. alba genomic DNA labeled with fluorescein isothiocyanate.
These two allohexaploids showed 24 hybridization signals demonstrating the presence of complete diploid chromosomes from S. alba and 36 chromosomes from B. juncea. The meiotic pollen mother cell showed 30 bivalent sets of all the 60 chromosomes and none of univalent or trivalent observed during meiosis.
The loss of function of HEL, which encodes a cellulose synthase interactive protein, causes helical and vine-like growth of tomato
Helical growth is an economical way for plant to obtain resources. The classic microtubule-microfibril alignment model of Arabidopsis helical growth involves restriction of the appropriate orientation of cellulose microfibrils appropriately in the cell walls. However, the molecular mechanism underlying tomato helical growth remains unknown. Here, we identified a spontaneous tomato helical (hel) mutant with right-handed helical cotyledons and petals but left-handed helical stems and true leaves.
Genetic analysis revealed that the hel phenotype was controlled by a single recessive gene. Using map-based cloning, we cloned the HEL gene, which encodes a cellulose interacting protein homologous to CSI1 of Arabidopsis. We identified a 27 bp fragment replacement that generated a premature stop codon. Transgenic experiments showed that the helical growth phenotype could be restored by the allele of this gene from wild-type Pyriforme. In contrast, the knockout mutation of HEL in Pyriforme via CRISPR/Cas9 resulted in helical growth. These findings shed light on the molecular control of the helical growth of tomato.
Many features that are appropriate for an ideal tissue engineered biomaterial are found in plant tissues. Hierarchically organized Bambusa vulgaris exhibits structural similarities to native bone, but the degradation of cellulose that is the main component of the plant cell wall is a challenge. In this study, Bamboo stem was subjected to decellularization followed by a chemical oxidation process (treated with sodium periodate) to enhance biocompatibility and biodegradation.
Green qPCR SuperMix |
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| abx098031-200l | Abbexa | 200 µl | EUR 912.5 |
Green qPCR SuperMix UDG |
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| abx098032-100l | Abbexa | 100 µl | EUR 687.5 |
Green qPCR SuperMix UDG |
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| 20-abx098032 | Abbexa |
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Green qPCR SuperMix UDG |
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| abx098032-200l | Abbexa | 200 µl | EUR 912.5 |
Tip Green qPCR SuperMix |
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| abx098034-100l | Abbexa | 100 µl | EUR 262.5 |
Tip Green qPCR SuperMix |
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| 20-abx098034 | Abbexa |
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Tip Green qPCR SuperMix |
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| abx098034-200l | Abbexa | 200 µl | EUR 437.5 |
Tip Green qPCR SuperMix (+Dye II) |
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| abx098891-100l | Abbexa | 100 µl | EUR 262.5 |
Tip Green qPCR SuperMix (+Dye II) |
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| 20-abx098891 | Abbexa |
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Tip Green qPCR SuperMix (+Dye II) |
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| abx098891-200l | Abbexa | 200 µl | EUR 437.5 |
EnTurbo™ SYBR Green PCR SuperMix |
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| EQ001 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix |
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| EQ001-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix |
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| EQ001-5mL | ELK Biotech | 5mL | EUR 80 |
Green Two-Step qRT-PCR SuperMix |
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| abx098035-100l | Abbexa | 100 µl | EUR 925 |
Green Two-Step qRT-PCR SuperMix |
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| abx098035-1ml | Abbexa | 1 ml | Ask for price |
Green Two-Step qRT-PCR SuperMix |
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| abx098035-200l | Abbexa | 200 µl | Ask for price |
Green Two-Step qRT-PCR SuperMix |
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| abx098035-50rxns20ulRTSystems300rxns20ulqPCRSystems | Abbexa | 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) | EUR 777.6 |
Green One-Step qRT-PCR SuperMix |
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| abx098038-100l | Abbexa | 100 µl | EUR 737.5 |
Green One-Step qRT-PCR SuperMix |
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| 20-abx09803820ulSystems | Abbexa |
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Green One-Step qRT-PCR SuperMix |
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| abx098038-1ml | Abbexa | 1 ml | Ask for price |
Green One-Step qRT-PCR SuperMix |
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| abx098038-200l | Abbexa | 200 µl | EUR 1050 |
Easy-LoadTM PCR SuperMix (Green, 2X) |
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| D7256-1ml | Beyotime Biotech Inc | 100 Tests | Ask for price |
Easy-LoadTM PCR SuperMix (Green, 2X) |
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| D7256-20ml | Beyotime Biotech Inc | 2000 Tests | Ask for price |
Easy-LoadTM PCR SuperMix (Green, 2X) |
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| D7256-4ml | Beyotime Biotech Inc | 400 Tests | Ask for price |
Easy-LoadTM PCR SuperMix (Green, 2X) |
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| D7256FT | Beyotime Biotech Inc | 20 Tests | Ask for price |
Green miRNA Two-Step qRT-PCR SuperMix |
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| abx098036-100l | Abbexa | 100 µl | EUR 1212.5 |
Green miRNA Two-Step qRT-PCR SuperMix |
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| abx098036-1ml | Abbexa | 1 ml | Ask for price |
Green miRNA Two-Step qRT-PCR SuperMix |
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| abx098036-200l | Abbexa | 200 µl | Ask for price |
Green miRNA Two-Step qRT-PCR SuperMix |
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| abx098036-20rxns20ulRTSystems500rxns20ulqPCRSystems | Abbexa | 20 rxns × 20 ul (RTSystems) / 500 rxns × 20 ul (qPCRSystems) | EUR 1062 |
Green Two-Step qRT-PCR SuperMix (GC Rich) |
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| abx098037-100l | Abbexa | 100 µl | EUR 1125 |
Green Two-Step qRT-PCR SuperMix (GC Rich) |
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| abx098037-1ml | Abbexa | 1 ml | Ask for price |
Green Two-Step qRT-PCR SuperMix (GC Rich) |
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| abx098037-200l | Abbexa | 200 µl | Ask for price |
Green Two-Step qRT-PCR SuperMix (GC Rich) |
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| abx098037-50rxns20ulRTSystems300rxns20ulqPCRSystems | Abbexa | 50 rxns × 20 ul (RTSystems) / 300 rxns × 20 ul (qPCRSystems) | EUR 961.2 |
Green One-Step qRT-PCR SuperMix (GC Rich) |
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| abx098039-100l | Abbexa | 100 µl | EUR 450 |
Green One-Step qRT-PCR SuperMix (GC Rich) |
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| 20-abx09803920ulSystems | Abbexa |
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Green One-Step qRT-PCR SuperMix (GC Rich) |
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| abx098039-1ml | Abbexa | 1 ml | Ask for price |
Green One-Step qRT-PCR SuperMix (GC Rich) |
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| abx098039-200l | Abbexa | 200 µl | EUR 1000 |
Probe qPCR SuperMix |
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| 20-abx098040 | Abbexa |
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EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed) |
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| EQ014 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed) |
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| EQ014-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed) |
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| EQ014-5mL | ELK Biotech | 5mL | EUR 80 |
EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed) |
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| EQ013 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed) |
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| EQ013-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed) |
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| EQ013-5mL | ELK Biotech | 5mL | EUR 80 |
Library Quantification qPCR SuperMix |
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| abx098896-100l | Abbexa | 100 µl | EUR 700 |
Library Quantification qPCR SuperMix |
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| abx098896-200l | Abbexa | 200 µl | EUR 962.5 |
Library Quantification qPCR SuperMix |
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| 20-abx098896 | Abbexa |
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EnTurbo™ probe qPCR SuperMix |
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| EQ017 | ELK Biotech | 5mL | EUR 240 |
EnTurbo™ probe qPCR SuperMix |
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| EQ017-25mL | ELK Biotech | 25mL | EUR 240 |
EnTurbo™ probe qPCR SuperMix |
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| EQ017-5mL | ELK Biotech | 5mL | EUR 78 |
cDNA Synthesis SuperMix for qPCR |
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| abx098019-100l | Abbexa | 100 µl | EUR 825 |
cDNA Synthesis SuperMix for qPCR |
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| 20-abx09801920ulSystems | Abbexa |
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cDNA Synthesis SuperMix for qPCR |
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| abx098019-1ml | Abbexa | 1 ml | EUR 2387.5 |
cDNA Synthesis SuperMix for qPCR |
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| abx098019-200l | Abbexa | 200 µl | EUR 1000 |
EnTurbo™ SYBR Color qPCR SuperMix |
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| EQ036 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix |
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| EQ036-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix |
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| EQ036-5mL | ELK Biotech | 5mL | EUR 80 |
HyperScript™ RT SuperMix for qPCR |
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| K1074-100 | ApexBio | 100 rxn (20 uL/rxn) | EUR 296 |
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Description: Reverse Transcription & PCR|Reverse Transcription |
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HyperScript™ RT SuperMix for qPCR |
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| K1074-50 | ApexBio | 50 rxn (20 uL/rxn) | EUR 166.4 |
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Description: Reverse Transcription & PCR|Reverse Transcription |
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HiScript II Q RT SuperMix for qPCR |
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| R222-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 145 |
Malachite Green Phosphate Assay kit |
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| 65-POMG-25H | Gentaur Genprice | 2500 tests | EUR 333 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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| abx098024-100l | Abbexa | 100 µl | EUR 1025 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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| abx098024-1ml | Abbexa | 1 ml | Ask for price |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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| abx098024-200l | Abbexa | 200 µl | Ask for price |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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| abx098024-50rxns20ulSystems | Abbexa | 50 rxns × 20 ul Systems | EUR 861.6 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
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| EQ035 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
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| EQ035-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
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| EQ035-5mL | ELK Biotech | 5mL | EUR 80 |
HiScript II Q Select RT SuperMix for qPCR |
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| R232-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 145 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
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| R423-00-10rxns | Vazyme | 10 rxns | EUR 1.09 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
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| R423-01 | Vazyme | 100rxns(20µl/rxn) | EUR 326 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
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| R423-01-100rxns | Vazyme | 100 rxns | EUR 337.36 |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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| abx098860-100l | Abbexa | 100 µl | EUR 1012.5 |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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| abx098860-1ml | Abbexa | 1 ml | Ask for price |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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| abx098860-200l | Abbexa | 200 µl | Ask for price |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
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| R323-00-10rxns | Vazyme | 10 rxns | EUR 1.09 |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
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| R323-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 217.5 |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
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| R323-01-100rxns | Vazyme | 100 rxns | EUR 225.22 |
EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed) |
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| EQ033 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed) |
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| EQ033-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed) |
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| EQ033-5mL | ELK Biotech | 5mL | EUR 80 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
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| EQ034 | ELK Biotech | 5mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
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| EQ034-25mL | ELK Biotech | 25mL | EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
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| EQ034-5mL | ELK Biotech | 5mL | EUR 80 |
HiScript II Q RT SuperMix for qPCR (+gDNA wiper) |
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| R223-01 | Vazyme | 100rxns(20µl/rxn) | EUR 159.5 |
HyperScript™ RT SuperMix for qPCR (with gDNA wiper) |
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| K1174-100 | ApexBio | 100 rxns | EUR 320 |
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Description: Reverse Transcription & PCR|Reverse Transcription |
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HyperScript™ RT SuperMix for qPCR (with gDNA wiper) |
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| K1174-50 | ApexBio | 50 rxns | EUR 192 |
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Description: Reverse Transcription & PCR|Reverse Transcription |
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HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper) |
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| R233-01 | Vazyme | 100 rxn (20 μl/rxn) | EUR 159.5 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
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| R333-00-10rxns | Vazyme | 10 rxns | EUR 1.09 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
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| R333-01 | Vazyme | 100 rxns (21 μl/rxn) | EUR 217.5 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
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| R333-01-100rxns | Vazyme | 100 rxns | EUR 225.22 |
PCR SuperMix |
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| 20-abx098887 | Abbexa |
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Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus) |
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| 11141ES10 | Yeasen Biotechnology | 10T | EUR 25 |
Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus) |
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| 11141ES60 | Yeasen Biotechnology | 100T | EUR 230 |
2X PCR SuperMix |
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| MB200-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 11 |
2X PCR SuperMix |
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| MBS402025-100Reactions | MyBiosource | 100Reactions | EUR 175 |
2X PCR SuperMix |
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| MBS402025-4Reactions | MyBiosource | 4Reactions | EUR 160 |
2X PCR SuperMix |
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| MB200-P100S | Bio-Helix | 4 Reactions | Ask for price |
PCR SuperMix (-dye) |
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| abx098002-100l | Abbexa | 100 µl | EUR 237.5 |
PCR SuperMix (-dye) |
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| 20-abx098002 | Abbexa |
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PCR SuperMix (-dye) |
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| abx098002-200l | Abbexa | 200 µl | EUR 337.5 |
PCR SuperMix (+dye) |
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| abx098003-100l | Abbexa | 100 µl | EUR 237.5 |
PCR SuperMix (+dye) |
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| 20-abx098003 | Abbexa |
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PCR SuperMix (+dye) |
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| abx098003-200l | Abbexa | 200 µl | EUR 337.5 |
Plus PCR SuperMix |
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| abx098888-100l | Abbexa | 100 µl | EUR 300 |
Plus PCR SuperMix |
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| abx098888-200l | Abbexa | 200 µl | EUR 612.5 |
Plus PCR SuperMix |
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| 20-abx098888 | Abbexa |
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Evo? cDNA Supermix |
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| M1168-100 | Biovision | each | EUR 457.2 |
Evo? cDNA Supermix |
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| M1168-25 | Biovision | each | EUR 320.4 |
Novo? cDNA Supermix |
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| M1169-100 | Biovision | each | EUR 529.2 |
Novo? cDNA Supermix |
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| M1169-25 | Biovision | each | EUR 346.8 |
HotStar PCR Supermix |
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| MB201-0100 | GeneDireX | 100 Reactions | Ask for price |
Probe Direct SuperMix |
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| abx461057-1096tests | Abbexa | 10 × 96 tests | Ask for price |
Probe Direct SuperMix |
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| abx461057-596tests | Abbexa | 5 × 96 tests | EUR 2800 |
Probe Direct SuperMix |
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| abx461057-96tests | Abbexa | 96 tests | EUR 812.5 |
cDNA Synthesis SuperMix |
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| abx098014-100l | Abbexa | 100 µl | EUR 725 |
cDNA Synthesis SuperMix |
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| 20-abx09801420ulSystems | Abbexa |
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cDNA Synthesis SuperMix |
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| abx098014-1ml | Abbexa | 1 ml | Ask for price |
cDNA Synthesis SuperMix |
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| abx098014-200l | Abbexa | 200 µl | EUR 812.5 |
T PCR SuperMix (-dye) |
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| abx098005-100l | Abbexa | 100 µl | EUR 262.5 |
T PCR SuperMix (-dye) |
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| abx098005-200l | Abbexa | 200 µl | EUR 462.5 |
T PCR SuperMix (-dye) |
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| 20-abx098005 | Abbexa |
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T PCR SuperMix (+dye) |
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| abx098006-100l | Abbexa | 100 µl | EUR 262.5 |
T PCR SuperMix (+dye) |
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| abx098006-200l | Abbexa | 200 µl | EUR 462.5 |
T PCR SuperMix (+dye) |
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| 20-abx098006 | Abbexa |
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AmpHS 2X PCR SuperMix |
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| MB203-P100 | Bio-Helix | 100 rxns2 x 1.25 mL | EUR 11 |
AmpHS 2X PCR SuperMix |
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| MB203-P040 | Bio-Helix | 40 Reactions | Ask for price |
EasyPfu PCR SuperMix (-dye) |
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| 20-abx098009 | Abbexa |
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FastPfu PCR SuperMix (-dye) |
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| abx098010-100l | Abbexa | 100 µl | EUR 300 |
FastPfu PCR SuperMix (-dye) |
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| abx098010-200l | Abbexa | 200 µl | EUR 575 |
FastPfu PCR SuperMix (-dye) |
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| 20-abx098010 | Abbexa |
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Library Amplification SuperMix |
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| abx098894-100l | Abbexa | 100 µl | EUR 737.5 |
Library Amplification SuperMix |
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| abx098894-200l | Abbexa | 200 µl | EUR 1137.5 |
Library Amplification SuperMix |
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| 20-abx098894 | Abbexa |
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OneScriptcDNA Synthesis SuperMix |
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| G451 | ABM | 25 x 20 ul reactions | EUR 106.8 |
OneScriptcDNA Synthesis SuperMix |
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| G452 | ABM | 100 x 20 ul reactions | EUR 169.2 |
PCR SuperMix for PAGE (+dye) |
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| abx098004-100l | Abbexa | 100 µl | EUR 237.5 |
PCR SuperMix for PAGE (+dye) |
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| 20-abx098004 | Abbexa |
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PCR SuperMix for PAGE (+dye) |
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| abx098004-200l | Abbexa | 200 µl | EUR 337.5 |
OneScriptPlus cDNA Synthesis SuperMix |
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| G453 | ABM | 25 x 20 ul reactions | EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
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| G454 | ABM | 100 x 20 ul reactions | EUR 202.8 |
OmniPCR Supermix w Fluorescent dye |
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| MBA01-0100 | Bio-Helix | 100 rxns (2.5ml) | EUR 16 |
OmniPCR Supermix w Fluorescent dye |
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| MBA01-0100S | Bio-Helix | 4 Reactions | Ask for price |
The crystallinity of oxidised plant scaffolds was reduced, resulting in lower mechanical strength. In contrast, hydrophilicity was enhanced in those scaffolds.