Small diameter branchlets and easy barked stems and branches of most woody vegetation have chloroplasts. Whereas the stems of a number of eucalypt species have been proven to photosynthesise, the distribution of chloroplasts has not been investigated intimately. The distribution of chloroplasts in branchlets (23 species) and bigger diameter stems and branches with easy bark (14 species) was investigated in a variety of eucalypts (species of Angophora, Corymbia and Eucalyptus) utilizing recent hand sections and a mixture of shiny subject and fluorescence microscopy.
All species had ample stem chloroplasts. In each small and enormous diameter stems, the best focus of chloroplasts was in a slim band (often 100-300 μm thick) instantly beneath the dermis or phellem. Deeper chloroplasts had been current however at a decrease density resulting from ample fibres and sclereids. Usually, chloroplasts had been discovered at higher depths in small diameter stems, typically being current within the secondary xylem rays and the pith. The cells of the chlorenchyma band had been small, rounded and densely packed, and in contrast to leaf mesophyll.
A excessive density of chloroplasts was discovered simply beneath the phellem of huge diameter stems. These bushes gave no exterior indication that inexperienced tissues had been current slightly below the phellem. In these species, a thick phellem was not current to guard the internal residing bark. Together with the chlorenchyma, the outer bark additionally had a excessive density of fibres and sclereids. These sclerenchyma cells most likely disrupted a higher abundance and a extra organised association of the cells containing chloroplasts. This exhibits a doable trade-off between photosynthesis and the standard bark capabilities of safety and mechanical power.
Spinach and Chive for Kidney Tubule Engineering: the Limitations of Decellularized Plant Scaffolds and Vasculature
Tissue decellularization yields advanced scaffolds with retained composition and construction, and vegetation supply an inexhaustible pure supply of quite a few shapes. Plant tissue may very well be an answer for regenerative organ alternative methods and superior in vitro modeling, as biofunctionalization of decellularized tissue permits adhesion of assorted sorts of human cells that may develop into useful tissue. Right here, we investigated the potential of spinach leaf vasculature and chive stems for kidney tubule engineering to use in tubular transport research.
We efficiently decellularized each plant tissues and confirmed normal scaffold suitability for topical recellularization with renal cells. Nonetheless, resulting from anatomical restrictions, we imagine that spinach and chive vasculature themselves can’t be recellularized by present strategies. Furthermore, gradual tissue disintegration and poor diffusion capability make decellularized plant scaffolds unsuitable for kidney tubule engineering, which depends on transepithelial solute change between two compartments. We conclude that plant-derived buildings and biomaterials should be rigorously thought of and probably built-in with different tissue engineering applied sciences for enhanced capabilities.
Response wooden (RW) formation is an innate physiological response of woody vegetation to counteract mechanical constraints in nature, reinforce construction and redirect progress towards the vertical course. Variations and/or similarities between stem and root response to mechanical constraints stay virtually unknown particularly in relation to phytohormones distribution and RW traits. Thus, Populus nigra stem and root subjected to static non-destructive mid-term bending therapy had been analyzed. The distribution of pressure and compression forces was firstly modeled alongside the primary bent stem and root axis; then, anatomical options, chemical composition, and an entire auxin and cytokinin metabolite profiles of the stretched convex and compressed concave aspect of three totally different bent stem and root sectors had been analyzed. The outcomes confirmed that in bent stems RW was produced on the higher stretched convex aspect whereas in bent roots it was produced on the decrease compressed concave aspect.
Metabolite Profile of Xylem Sap in Cotton Seedlings Is Modified by Ok Deficiency
Xylem sap, belonging to the plant apoplast, not solely supplies plant tissues with inorganic and natural substances but additionally facilitates communication between the roots and the leaves and coordinates their improvement. This research investigated the consequences of potassium (Ok) deficiency on the morphology and the physiology of cotton seedlings in addition to pH, mineral nutrient contents, and metabolites of xylem sap. Particularly, we in contrast modifications in root-shoot communication below low Ok (LK) and regular Ok (NK, management) ranges.
In comparison with management, LK stress considerably decreased seedling biomass (leaf, stem, and root dry weight; stem and root size; root floor space and root quantity) and the degrees of Ok, Na (sodium), Mg (magnesium), Fe (iron), and Zn in xylem sap. A complete of 82 metabolites in sap analyzed by high-performance liquid chromatography-tandem mass spectrometry confirmed important variations between the 2 circumstances; amongst these, 38 had been up-regulated greater than 2-fold, whereas the others had been down-regulated lower than 0.5-fold.
Particularly, a number of metabolites discovered within the cell membrane together with three cholines and desglucocoroloside and others akin to malondialdehyde, α-amino acids and derivatives, sucrose, and sugar alcohol considerably elevated below LK stress, indicating that cell membranes had been broken and protein metabolism was irregular. It’s value noting that glycerophosphocholine was up-regulated 29-fold below LK stress, indicating that it may be used as an essential sign of root-shoot communication.
Moreover, in pathway analyses, 26 metabolites had been matched to Kyoto Encyclopedia of Genes and Genomes pathways; L-aspartic acid, which was related to 10 KEGG pathways, was essentially the most concerned metabolite. Total, Ok deficiency diminished the antioxidant capability of cotton seedlings and led to a metabolic dysfunction together with elevated ranges of main metabolites and inhibited manufacturing of secondary metabolites.
) EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
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EQ034-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (High ROX Premixed) |
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EQ034-5mL |
ELK Biotech |
5mL |
EUR 80 |
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EQ014 |
ELK Biotech |
5mL |
EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed) |
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EQ014-25mL |
ELK Biotech |
25mL |
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EnTurbo™ SYBR Green PCR SuperMix(Low ROX Premixed) |
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EQ014-5mL |
ELK Biotech |
5mL |
EUR 80 |
) EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed) |
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EQ013 |
ELK Biotech |
5mL |
EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed) |
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EQ013-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Green PCR SuperMix(High ROX Premixed) |
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EQ013-5mL |
ELK Biotech |
5mL |
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Q331-02 |
Vazyme |
500 rxns (20 μl/rxn) |
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ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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Q331-02-500rxns |
Vazyme |
500 rxns |
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ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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Q331-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
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ChamQ SYBR qPCR Master Mix (Low ROX Premixed) |
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Q331-03-2500rxns |
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2,500 rxns |
EUR 562.79 |
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Q341-02 |
Vazyme |
500 rxns (20 μl/rxn) |
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ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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Q341-02-500rxns |
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500 rxns |
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ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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Q341-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 543.5 |
ChamQ SYBR qPCR Master Mix (High ROX Premixed) |
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Q341-03-2500rxns |
Vazyme |
2,500 rxns |
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Q431-02 |
Vazyme |
500 rxns (20 μl/rxn) |
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ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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Q431-02-500rxns |
Vazyme |
500 rxns |
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ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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Q431-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed) |
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Q431-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 656.51 |
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Q441-02 |
Vazyme |
500 rxns (20 μl/rxn) |
EUR 140.63 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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Q441-02-500rxns |
Vazyme |
500 rxns |
EUR 145.62 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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Q441-03 |
Vazyme |
2,500 rxns (20 μl/rxn) |
EUR 634 |
ChamQ SYBR Color qPCR Master Mix (High ROX Premixed) |
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Q441-03-2500rxns |
Vazyme |
2,500 rxns |
EUR 656.51 |
) Genious 2X SYBR Green Fast qPCR Mix (Low ROX Premixed) |
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RK21206 |
Abclonal |
1 ml |
EUR 76.3 |
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) Genious 2X SYBR Green Fast qPCR Mix (High ROX Premixed) |
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RK21207 |
Abclonal |
1 ml |
EUR 21.26 |
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) EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
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EQ035 |
ELK Biotech |
5mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
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EQ035-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix (Without ROX) |
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EQ035-5mL |
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20-abx098040 |
Abbexa |
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 Green qPCR SuperMix |
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abx098031-100l |
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Abbexa |
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Green qPCR SuperMix |
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abx098031-200l |
Abbexa |
200 µl |
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 Green qPCR SuperMix UDG |
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abx098032-100l |
Abbexa |
100 µl |
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Green qPCR SuperMix UDG |
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20-abx098032 |
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Green qPCR SuperMix UDG |
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abx098032-200l |
Abbexa |
200 µl |
EUR 912.5 |
 Top Green qPCR SuperMix |
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abx098033-100l |
Abbexa |
100 µl |
EUR 262.5 |
Top Green qPCR SuperMix |
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20-abx098033 |
Abbexa |
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Top Green qPCR SuperMix |
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abx098033-200l |
Abbexa |
200 µl |
EUR 487.5 |
 Tip Green qPCR SuperMix |
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abx098034-100l |
Abbexa |
100 µl |
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Tip Green qPCR SuperMix |
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20-abx098034 |
Abbexa |
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Tip Green qPCR SuperMix |
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abx098034-200l |
Abbexa |
200 µl |
EUR 437.5 |
 EnTurbo™ probe qPCR SuperMix |
|
EQ017 |
ELK Biotech |
5mL |
EUR 240 |
EnTurbo™ probe qPCR SuperMix |
|
EQ017-25mL |
ELK Biotech |
25mL |
EUR 240 |
EnTurbo™ probe qPCR SuperMix |
|
EQ017-5mL |
ELK Biotech |
5mL |
EUR 78 |
 Library Quantification qPCR SuperMix |
|
abx098896-100l |
Abbexa |
100 µl |
EUR 700 |
Library Quantification qPCR SuperMix |
|
abx098896-200l |
Abbexa |
200 µl |
EUR 962.5 |
Library Quantification qPCR SuperMix |
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20-abx098896 |
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) Top Green qPCR SuperMix (+Dye II) |
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abx098890-100l |
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100 µl |
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Top Green qPCR SuperMix (+Dye II) |
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abx098890-200l |
Abbexa |
200 µl |
EUR 487.5 |
) Tip Green qPCR SuperMix (+Dye II) |
|
abx098891-100l |
Abbexa |
100 µl |
EUR 262.5 |
Tip Green qPCR SuperMix (+Dye II) |
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20-abx098891 |
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Tip Green qPCR SuperMix (+Dye II) |
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abx098891-200l |
Abbexa |
200 µl |
EUR 437.5 |
 EnTurbo™ SYBR Color qPCR SuperMix |
|
EQ036 |
ELK Biotech |
5mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix |
|
EQ036-25mL |
ELK Biotech |
25mL |
EUR 340 |
EnTurbo™ SYBR Color qPCR SuperMix |
|
EQ036-5mL |
ELK Biotech |
5mL |
EUR 80 |
 cDNA Synthesis SuperMix for qPCR |
|
abx098019-100l |
Abbexa |
100 µl |
EUR 825 |
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20-abx09801920ulSystems |
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cDNA Synthesis SuperMix for qPCR |
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abx098019-1ml |
Abbexa |
1 ml |
EUR 2387.5 |
cDNA Synthesis SuperMix for qPCR |
|
abx098019-200l |
Abbexa |
200 µl |
EUR 1000 |
 1ml PrecisionPLUS qPCR Master Mix premixed with SYBRgreen |
|
Z-PPLUS-SY-1ML |
Novacyt Group |
1ml |
EUR 81 |
|
Description: Precision®PLUS |
 HyperScript™ RT SuperMix for qPCR |
|
K1074-100 |
ApexBio |
100 rxn (20 uL/rxn) |
EUR 296 |
|
Description: Reverse Transcription & PCR|Reverse Transcription |
HyperScript™ RT SuperMix for qPCR |
|
K1074-50 |
ApexBio |
50 rxn (20 uL/rxn) |
EUR 166.4 |
|
Description: Reverse Transcription & PCR|Reverse Transcription |
 1ml Precision FAST qPCR Master Mix premixed with SYBRgreen |
|
Z-PFAST-SY-1ML |
Novacyt Group |
1ml |
EUR 90 |
|
Description: Precision®FAST |
 2ml Precision FAST qPCR Master Mix premixed with SYBRgreen |
|
Z-PFAST-SY-2ML |
Novacyt Group |
2ml |
EUR 161 |
|
Description: Precision®FAST |
 HiScript II Q RT SuperMix for qPCR |
|
R222-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 145 |
) VAHTS Library Quantification Kit for Illumina (Low ROX Premixed) |
|
NQ103 |
Vazyme |
500 rxn |
EUR 349.2 |
VAHTS Library Quantification Kit for Illumina (Low ROX Premixed) |
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NQ103-500rxns |
Vazyme |
500 rxns |
EUR 181.21 |
) VAHTS Library Quantification Kit for Illumina (High ROX Premixed) |
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NQ104 |
Vazyme |
500 rxn |
EUR 349.2 |
) VAHTS Library Quantification Kit for IIIumina (High ROX Premixed) |
|
NQ104-500rxns |
Vazyme |
500 rxns |
EUR 181.21 |
) cDNA Synthesis SuperMix for qPCR (GC-rich) |
|
abx098024-100l |
Abbexa |
100 µl |
EUR 1025 |
cDNA Synthesis SuperMix for qPCR (GC-rich) |
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abx098024-1ml |
Abbexa |
1 ml |
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cDNA Synthesis SuperMix for qPCR (GC-rich) |
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abx098024-200l |
Abbexa |
200 µl |
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cDNA Synthesis SuperMix for qPCR (GC-rich) |
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abx098024-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 861.6 |
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 HiScript II Q Select RT SuperMix for qPCR |
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R232-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 145 |
) HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
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R423-00-10rxns |
Vazyme |
10 rxns |
EUR 1.09 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
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R423-01 |
Vazyme |
100rxns(20µl/rxn) |
EUR 326 |
HiScript IV RT SuperMix for qPCR (+gDNA wiper) |
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R423-01-100rxns |
Vazyme |
100 rxns |
EUR 337.36 |
 Uni Cell to cDNA Synthesis SuperMix for qPCR |
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abx098860-100l |
Abbexa |
100 µl |
EUR 1012.5 |
Uni Cell to cDNA Synthesis SuperMix for qPCR |
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abx098860-1ml |
Abbexa |
1 ml |
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Uni Cell to cDNA Synthesis SuperMix for qPCR |
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abx098860-200l |
Abbexa |
200 µl |
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) HiScript III RT SuperMix for qPCR (+gDNA wiper) |
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R323-00-10rxns |
Vazyme |
10 rxns |
EUR 1.09 |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
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R323-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 217.5 |
HiScript III RT SuperMix for qPCR (+gDNA wiper) |
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R323-01-100rxns |
Vazyme |
100 rxns |
EUR 225.22 |
) HiScript II Q RT SuperMix for qPCR (+gDNA wiper) |
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R223-01 |
Vazyme |
100rxns(20µl/rxn) |
EUR 159.5 |
) HyperScript™ RT SuperMix for qPCR (with gDNA wiper) |
|
K1174-100 |
ApexBio |
100 rxns |
EUR 320 |
|
Description: Reverse Transcription & PCR|Reverse Transcription |
HyperScript™ RT SuperMix for qPCR (with gDNA wiper) |
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K1174-50 |
ApexBio |
50 rxns |
EUR 192 |
|
Description: Reverse Transcription & PCR|Reverse Transcription |
) HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper) |
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R233-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 159.5 |
 Pro-QPCR-SuperMix-Kit---ROX-premixed |
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K5056200 |
Biochain |
200 reactions |
EUR 126 |
Pro-QPCR-SuperMix-Kit---ROX-premixed |
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K5056400 |
Biochain |
400 reactions |
EUR 228.2 |
 HiScript III All-in-one RT SuperMix Perfect for qPCR |
|
R333-00-10rxns |
Vazyme |
10 rxns |
EUR 1.09 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
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R333-01 |
Vazyme |
100 rxns (21 μl/rxn) |
EUR 217.5 |
HiScript III All-in-one RT SuperMix Perfect for qPCR |
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R333-01-100rxns |
Vazyme |
100 rxns |
EUR 225.22 |
 Fast-Pro-QPCR-SuperMix-Kit---ROX-premixed |
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K5058200 |
Biochain |
200 reactions |
EUR 147.7 |
Fast-Pro-QPCR-SuperMix-Kit---ROX-premixed |
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K5058400 |
Biochain |
400 reactions |
EUR 247.8 |
) Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus) |
|
11141ES10 |
Yeasen Biotechnology |
10T |
EUR 25 |
Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus) |
|
11141ES60 |
Yeasen Biotechnology |
100T |
EUR 230 |
 Animal Detection Probe qPCR Super PreMix |
|
QV114-EN01 |
Vazyme |
400 rxns |
EUR 160 |
Animal Detection Probe qPCR Super PreMix |
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QV114-EN01-400rxns |
Vazyme |
400 rxns |
EUR 165.68 |
Animal Detection Probe qPCR Super PreMix |
|
QV114-EN02 |
Vazyme |
800 rxns |
EUR 288 |
Animal Detection Probe qPCR Super PreMix |
|
QV114-EN02-800rxns |
Vazyme |
800 rxns |
EUR 298.22 |
 PCR SuperMix |
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20-abx098887 |
Abbexa |
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 Acryl/Bis 37.5:1 Premixed Powder, 200g |
|
PR0604-200g |
Vivantis |
each |
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PR0604-40g |
Vivantis |
each |
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 2X PCR SuperMix |
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MB200-P100 |
Bio-Helix |
100 rxns2 x 1.25 mL |
EUR 11 |
2X PCR SuperMix |
|
MBS402025-100Reactions |
MyBiosource |
100Reactions |
EUR 175 |
2X PCR SuperMix |
|
MBS402025-4Reactions |
MyBiosource |
4Reactions |
EUR 160 |
 Probe Direct SuperMix |
|
abx461057-1096tests |
Abbexa |
10 × 96 tests |
Ask for price |
Probe Direct SuperMix |
|
abx461057-596tests |
Abbexa |
5 × 96 tests |
EUR 2800 |
Probe Direct SuperMix |
|
abx461057-96tests |
Abbexa |
96 tests |
EUR 812.5 |
) PCR SuperMix (-dye) |
|
abx098002-100l |
Abbexa |
100 µl |
EUR 237.5 |
PCR SuperMix (-dye) |
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20-abx098002 |
Abbexa |
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PCR SuperMix (-dye) |
|
abx098002-200l |
Abbexa |
200 µl |
EUR 337.5 |
) PCR SuperMix (+dye) |
|
abx098003-100l |
Abbexa |
100 µl |
EUR 237.5 |
PCR SuperMix (+dye) |
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20-abx098003 |
Abbexa |
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PCR SuperMix (+dye) |
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abx098003-200l |
Abbexa |
200 µl |
EUR 337.5 |
 Plus PCR SuperMix |
|
abx098888-100l |
Abbexa |
100 µl |
EUR 300 |
Plus PCR SuperMix |
|
abx098888-200l |
Abbexa |
200 µl |
EUR 612.5 |
Plus PCR SuperMix |
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20-abx098888 |
Abbexa |
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 Evo? cDNA Supermix |
|
M1168-100 |
Biovision |
each |
EUR 457.2 |
Evo? cDNA Supermix |
|
M1168-25 |
Biovision |
each |
EUR 320.4 |
 Novo? cDNA Supermix |
|
M1169-100 |
Biovision |
each |
EUR 529.2 |
Novo? cDNA Supermix |
|
M1169-25 |
Biovision |
each |
EUR 346.8 |
 cDNA Synthesis SuperMix |
|
abx098014-100l |
Abbexa |
100 µl |
EUR 725 |
cDNA Synthesis SuperMix |
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20-abx09801420ulSystems |
Abbexa |
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cDNA Synthesis SuperMix |
|
abx098014-1ml |
Abbexa |
1 ml |
Ask for price |
cDNA Synthesis SuperMix |
|
abx098014-200l |
Abbexa |
200 µl |
EUR 812.5 |
) T PCR SuperMix (-dye) |
|
abx098005-100l |
Abbexa |
100 µl |
EUR 262.5 |
T PCR SuperMix (-dye) |
|
abx098005-200l |
Abbexa |
200 µl |
EUR 462.5 |
T PCR SuperMix (-dye) |
|
20-abx098005 |
Abbexa |
-
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) T PCR SuperMix (+dye) |
|
abx098006-100l |
Abbexa |
100 µl |
EUR 262.5 |
T PCR SuperMix (+dye) |
|
abx098006-200l |
Abbexa |
200 µl |
EUR 462.5 |
T PCR SuperMix (+dye) |
|
20-abx098006 |
Abbexa |
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 AmpHS 2X PCR SuperMix |
|
MB203-P100 |
Bio-Helix |
100 rxns2 x 1.25 mL |
EUR 11 |
 qPCR PreMix Kit |
|
MB1001-2 |
BosterBio |
1 Kit |
EUR 276 |
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Description: qPCR PreMix is a kit for providing accurate data from your various samples speedily. There are no additional mixing steps because it already prepared and dried for your convenient. This product makes you monitor your gene amplification of Ct in every steps of amplified florescent cycle because we add “probe” which include florescence oligo-nucleotide and primers. |
) EasyPfu PCR SuperMix (-dye) |
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20-abx098009 |
Abbexa |
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) FastPfu PCR SuperMix (-dye) |
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abx098010-100l |
Abbexa |
100 µl |
EUR 300 |
FastPfu PCR SuperMix (-dye) |
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abx098010-200l |
Abbexa |
200 µl |
EUR 575 |
FastPfu PCR SuperMix (-dye) |
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20-abx098010 |
Abbexa |
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 Library Amplification SuperMix |
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abx098894-100l |
Abbexa |
100 µl |
EUR 737.5 |
Library Amplification SuperMix |
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abx098894-200l |
Abbexa |
200 µl |
EUR 1137.5 |
Library Amplification SuperMix |
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20-abx098894 |
Abbexa |
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 Premixed thermal fluid ready to use, box of 5 litres |
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CB-1002322 |
Phoenix instrument |
each |
EUR 81 |
 OneScriptcDNA Synthesis SuperMix |
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G451 |
ABM |
25 x 20 ul reactions |
EUR 106.8 |
This ultimately resulted in decreased biomass of cotton seedlings below LK stress. This research lays a stable basis for additional analysis on focused metabolites and sign substances within the xylem sap of cotton vegetation uncovered to Ok deficiency.
